Rat Mutations cvd and hob with Cerebellar Malformations Map to Chromosome 2

• KUWAMURA Mitsuru

  Laboratory of Veterinary Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• ANDO Yosuke

  Medicinal Safety Research Laboratories, Sankyo Co., Ltd.

• TAKADA Akiko

  Laboratory of Veterinary Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• KANEHARA Toshiko

  Laboratory of Veterinary Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• YAMATE Jyoji

  Laboratory of Veterinary Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• KOTANI Takao

  Laboratory of Veterinary Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• TAKESHITA Shigehito

  Medicinal Safety Research Laboratories, Sankyo Co., Ltd.

• KANBORI Miyuki

  Medicinal Safety Research Laboratories, Sankyo Co., Ltd.

• KITADA Kazuhiro

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• SERIKAWA Tadao

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

抄録

In this paper, we executed genome mapping and comparative mapping analyses for cvd and hob, autosomal recessive mutations with cerebellar vermis defect and cerebellar dysplasia in the rat. For the linkage analysis, we produced three sets of backcross progeny, (ACI × CVD)F₁ and (F344 × CVD)F₁ females crossed to a cvd homozygous male rat, and (HOB × WKY)F₁ males crossed to hob homozygous female rats. Analysis of the segregation patterns of simple sequence length polymorphism (SSLP) markers scanning the whole rat genome allowed the mapping of these autosomal recessive mutations to rat Chromosome (Chr) 2. The most likely gene order is D2Mgh12 - D2Rat86 - D2Mit15 - D2Rat185 - cvd - D2Rat66 - D2Mgh13, and D2Mit18 - Fga -D2Mit14 - D2Rat16 - hob - D2Mgh13. Crossing test between a proven cvd heterozygous and a hob heterozygous rats demonstrated their allelism. Furthermore, comparative mapping indicated the cvd locus corresponds to mouse chromosome 3 and a strong candidate gene Unc5h3, a causative gene for the rostral cerebellar malformation mouse, was implicated.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 53 (1), 21-26, 2004

  公益社団法人 日本実験動物学会

キーワード

• cerebellar vermis defect rat
• dysplasia
• neurological mutant
• hobble behavior rat
• rostral cerebellar malformation
• <i>Unc5h3</i>

詳細情報

• CRID: 1390001205042007552
• NII論文ID: 10011955911
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.53.21
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 6828634
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6828634
• Web Site: http://www.jstage.jst.go.jp/article/expanim/53/1/53_1_21/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2004217992
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可