界面特性を利用した細菌細胞の付着・脱着制御法

DOI Web Site 被引用文献2件 参考文献52件
  • 林 浩志
    早稲田大学　理工学部　応用化学科，日本学術振興会特別研究員PD (現　三菱マテリアル(株) 総合研究所　資源環境研究部)
  • 二瓶 智也
    早稲田大学大学院　理工学研究科　環境資源及材料理工学専攻(現　ライオン(株))
  • 生木 大志
    早稲田大学大学院　理工学研究科　環境資源及材料理工学専攻(現　サントリー(株))
  • 常田 聡
    早稲田大学　理工学部　応用化学科
  • 平田 彰
    早稲田大学　理工学部　応用化学科
  • 佐々木 弘
    早稲田大学　理工学部　環境資源工学科

書誌事項

タイトル別名
  • Control of Adhesion and Removal of Bacterial Cells from Stable Suspension Using Surface Characteristics
  • カイメン トクセイ オ リヨウ シタ サイキン サイボウ ノ フチャク ダッチャク セイギョホウ

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抄録

Rapid collection and removal of gram-negative heterotrophic bacterial strain, Escherichia coli, was investigated by utilizing the interfacial interaction between cell and solid substratum. Both E.coli collection from stable suspension and its successive removal were performed by a column bed packed with fibrous ferro-nickel slag, which was employed as the collector media of bacterial cells. In the cell collection tests, little cell recovery was obtained at neutral pH condition where both E.coli and FS had negative surface potential. On the other hand, E.coli collection gradually increased as the pH decreased. These findings were explained by the suppression of electrostatic repulsive interaction between E.coli and FS led to an improvement in cell attachment to FS surface. Cell collection capacity Γmax exponentially increased as the interfacial interaction energy minimum Vmin became lower, indicating that surface characteristics played crucial roles in cell attachment mechanism. Moreover, part of the E.coli cells that had adhered to FS surface were effectively removed when the eluting solutions were alkaline conditions; hence reversible cell detachment from FS was possible by controlling the electro-repulsive force. Cyclic E.coli collection/removal tests demonstrated that cell collection and successive removal were repeatedly carried out al least six times, although about 40 mg of E.coli cell made a firm and irreversible attachment on FS. E.coli collection/removal behaviors in the present experiments were generally in good agreement with electrokinetic properties of cell and FS, suggesting that cell recovery based on surface characteristics is a promising method, especially for stable bacterial suspension.

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  • 資源と素材

    資源と素材 120 (1), 54-59, 2004

    一般社団法人 資源・素材学会

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