D-バリン培地法により選択的に初代培養したラット胆管上皮細胞に対するPhospholipase A₂の細胞増殖促進効果の解析

• 小倉 薫

  香川医科大学小児外科学 香川医科大学生理学

• 渡辺 泰宏

  香川医科大学小児外科学

• 平峯 千春

  香川医科大学基礎看護学

• 中川 利孝

  香川医科大学附属実験実習機器センター

• 土岐 彰

  香川医科大学小児外科学

• 徳田 雅明

  香川医科大学生理学

書誌事項

タイトル別名

• Effect of phospholipase A₂ on proliferation of the primary cultured biliary epithelial cells from the extra-hepatic bile duct of rats using D-valine containing medium

抄録

We hypothesized that a high level of phospholipase A₂ (PLA₂) in bile juice works as a growth factor on biliary epithelial cells and causes hyperplastic change of the bile duct in pancreaticobiliary maljunction. In order to examine this hypothesis, we established a primary culture system of biliary epithelial cells from the extra-hepatic bile duct of rats. We successfully excluded fibroblasts from the epithelial cells using D-valine-containing medium and the single-cell cloning method. The established cells exhibited the characteristic features of rat bile duct epithelial cells. They were stained with anti-cytokeratin antibody, a marker protein of epithelial cells, were positive with PAS stain, and possessed the architectures of microvilli, tonofilament fibers and desmosomes. The effect of PLA₂ on proliferation of these cells was then examined. We clearly demonstrated that PLA₂ at nano-molar concentration (50nM, 100nM), that is, equivalent to the level of PLA₂ in the bile juice of pancreaticobiliary maljunction cases, caused a significant enhancement of epithelial cell proliferation.

収録刊行物

• 岡山医学会雑誌

  岡山医学会雑誌 114 (3), 289-296, 2003

  岡山医学会

キーワード

• 膵・胆管合流異常
• 胆管上皮細胞
• D-バリン
• 細胞増殖

詳細情報

• CRID: 1390282679853146624
• NII論文ID: 130006857958
• NII論文ID: 10012337225
• NII書誌ID: AN00032489
• DOI: 10.4044/joma1947.114.3_289
• ISSN: 18824528
• ISSN: 00301558
• Web Site: https://www.jstage.jst.go.jp/article/joma1947/114/3/114_3_289/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003248178
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可