除草剤による植物の光合成機能障害の画像診断 : クロロフィルα蛍光解析におけるインダクション法と飽和パルス法の比較 Image Diagnosis of Photosynthetic Injuries Induced by Herbicide in Plants : Comparison of the Induction Method with the Saturation Pulse Method for Chlorophyll α Fluorescence Analysis

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著者

    • 大政 謙次 OMASA Kenji
    • 東京大学大学院農学生命科学研究科 The University of Tokyo, Graduate School of Agricultural and Life Sciences
    • 後藤 英司 GOTO Eiji
    • 東京大学大学院農学生命科学研究科 The University of Tokyo, Graduate School of Agricultural and Life Sciences

抄録

We have developed a new image analysis system, capable of quantitative analysis of two aspects of chlorophyll a fluorescence ; i) the changes in intensity of chlorophyll fluorescence during dark-light transitions by the induction method, and ii) the chlorophyll fluorescence quenching by the saturation pulse method. With this system we analyzed the effects of an herbicide on the photosynthetic activity of <I>Phaseolus vulgaris</I> L. leaves. Furthermore, stomatal conductance and assimilation rate were measured with a diffusion porometer.<BR>Results showed that the photosynthetic injuries caused by the herbicide were detected by both fluorescence measurement methods. These injuries were also detected with the porometer as a decrease in stomatal conductance and assimilation rate. Information obtained by the induction method was effective for determining the site in the electron transport system which was damaged by the herbicide. The saturation pulse method could analyze the photosynthetic injuries more quantitatively than the induction method. However, the saturation pulse method required an evenly distributed high level of photosynthetic active radiation (PAR), which makes it difficult to apply to a large leaf area. In contrast, the induction method did not require such a high level of PAR for measurements, and was easier to use for a large leaf area. Consequently, simultaneous use of the saturation pulse method and the induction method enables detailed diagnosis of injuries of the photosynthetic system.

収録刊行物

  • 植物工場学会誌

    植物工場学会誌 13(1), 29-37, 2001-03-01

    JAPANESE SOCIETY OF AGRICULTURAL, BIOLOGICAL AND ENVIRONMENTAL ENGINEERS AND SCIENTISTS

参考文献:  27件中 1-27件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10012338724
  • NII書誌ID(NCID)
    AN10451761
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09186638
  • NDL 記事登録ID
    5687415
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z18-3397
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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