In Planta Transformation of Mulberry Trees (Morus alba L.) by Agrobacterium tumefaciens

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The <i>in planta</i> transformation method was used for mulberry plants [<i>Morus alba</i> L., F<SUB>1</SUB> generation obtained by natural pollination of a Chinese variety, Canzhuan No.4 (female)]. For transformation, an avirulent mutant of <i>Agrobacterium tumefaciens</i> (M-21), isolated by us, was used; the mutant is capable of integrating its T-DNA into chromosomes of host plants but produces no galls since a gene (tryptophan monooxygenase gene, <i>iaaM</i>) involved in auxin (indole acetic acid, IAA) biosynthesis in the T-DNA region is destructed by transposon5 (Tn<i>5</i>) insertion. All other genes including the <i>ipt</i> gene for cytokinin biosynthesis in the T-DNA region are intact. Young mulberry trees in pots were decapitated and meristems of axillary buds were inoculated by water suspension of <i>A. tumefaciens</i> after being pricked with a needle. The inoculated four plants were allowed to grow in pots. All of the four transformants in the T<SUB>0</SUB> generation showed characteristic phenotypes different from those of non-transformed plants, which seemed to be due to overproduction of cytokinin. The transgene (Tn5-inserted T-DNA) was detected in all of four transformants by nested PCR. The offspring of transformants (T<SUB>0</SUB>) produced by stem cuttage also showed remarkable phenotypes different from those of offspring of non-transformed plants. The transgene was transmitted in all of the offspring of transformants. Thus, the transgenic mulberry plants can be produded simply and efficiently by the <i>in planta</i> transformation method as detailed in this paper.

収録刊行物

  • Journal of insect biotechnology and sericology

    Journal of insect biotechnology and sericology 72(3), 177-184, 2003-10-31

    社団法人 日本蚕糸学会

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被引用文献:  4件中 1-4件 を表示

各種コード

  • NII論文ID(NAID)
    10012453412
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    6730866
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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