ウサギとハムスターの卵母細胞の成熟に伴うヒドロキシステロイド脱水素酵素活性の変化 Changes in the Activities of Hydroxysteroid Dehydrogenases in Rabbit and Hamster Oocytes during Meiotic Maturation

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The activities of hydroxysteroid dehydrogenases (HSDs) were histochemically demonstrated in rabbit and hamster oocytes in the process of maturation, and the changes in steroid metabolism during meiotic maturation were examined. In rabbits, the percentages of oocytes showing the activities of Δ5-3β-HSD (with DHEA as the substrate), 17β-HSD (testosterone) and 20β-HSD (20β-hydroxyprogesterone) were always high and did not change during maturation, whereas those showing the activities of Δ5-3β-HSD (pregnenolone and 17α-hydroxy-pregnenolone), 17 β-HSD (estradiol-17β), 20α-HSD (20 α-hydroxyprogesterone) and 20β-HSD (17α-hydroxyprogesterone) decreased as the time after the hCG injection was prolonged. On the other hand, the activities of Δ5-3β-HSD, 17 β-HSD, 20α-HSD and 20β-HSD with eight substrates were almost always observed in hamster oocytes from 0 to 13 hrs after the hCG injection. The present findings suggested that the metabolic abilities of 20β-hydroxyprogesterone and androgen are constantly present in rabbit oocytes in the process of maturation, whereas those of progesterone, 17α-hydroxyprogesterone, 17α, 20β-dihydroxyprogesterone, 20α-hydroxyprogesterone and estrogen decrease during maturation. And it was also confirmed in hamster oocytes that the metabolic abilities of progesterone, 17α-hydroxyprogesterone, 17α, 20β-dihydroxyprogesterone, 20α-hydroxyprogesterone, 20β-hydroxyprogesterone, estrogen and androgen are always present and do not vary with maturation.

The activities of hydroxysteroid dehydrogenases (HSDs) were histochemically demonstrated in rabbit and hamster oocytes in the process of maturation, and the changes in steroid metabolism during meiotic maturation were examined. In rabbits, the percentages of oocytes showing the activities of Δ<sup>5</sup>-3β-HSD (with DHEA as the substrate), 17β-HSD (testosterone) and 20β-HSD (20β-hydroxyprogesterone) were always high and did not change during maturation, whereas those showing the activities of Δ<sup>5</sup>-3β-HSD (pregnenolone and 17α-hydroxy-pregnenolone), 17 β-HSD (estradiol-17β), 20α-HSD (20 α-hydroxyprogesterone) and 20β-HSD (17α-hydroxyprogesterone) decreased as the time after the hCG injection was prolonged. On the other hand, the activities of Δ<sup>5</sup>-3β-HSD, 17 β-HSD, 20α-HSD and 20β-HSD with eight substrates were almost always observed in hamster oocytes from 0 to 13 hrs after the hCG injection. The present findings suggested that the metabolic abilities of 20β-hydroxyprogesterone and androgen are constantly present in rabbit oocytes in the process of maturation, whereas those of progesterone, 17α-hydroxyprogesterone, 17α, 20β-dihydroxyprogesterone, 20α-hydroxyprogesterone and estrogen decrease during maturation. And it was also confirmed in hamster oocytes that the metabolic abilities of progesterone, 17α-hydroxyprogesterone, 17α, 20β-dihydroxyprogesterone, 20α-hydroxyprogesterone, 20β-hydroxyprogesterone, estrogen and androgen are always present and do not vary with maturation.<br>

収録刊行物

  • Journal of mammalian ova research = 日本哺乳動物卵子学会誌

    Journal of mammalian ova research = 日本哺乳動物卵子学会誌 20(3), 118-123, 2003-10-01

    日本哺乳動物卵子学会

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各種コード

  • NII論文ID(NAID)
    10012455121
  • NII書誌ID(NCID)
    AN10548943
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417738
  • データ提供元
    CJP書誌  IR  J-STAGE 
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