Granule-bound Starch Synthase I Isozyme Localized Predominantly in Kidney Bean Leaves

• Matsui Hirokazu

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• Senoura Takeshi

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• Isono Naoto

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• Sakurai Yasutaka

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• Hamada Shigeki

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• Ito Hiroyuki

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

Bibliographic Information

Other Title

• インゲンマメ緑葉に存在するGranule-bound
• Granule-bound Starch Synthase 1 Isozyme Localized Predominantly in Kidney Bean Leaves

Abstract

Abstract: Starch granules from the leaves of kidney bean (Phaseolus vulgaris L.), as well as from seeds, asso ciate the protein with the molecular mass of 59 kDa. Our previous study has shown that the 59 kDa-protein in seeds is granule-bound starch synthase I (PvGBSSIa) and that the transcript specifically accumulates to seeds. The determined N-terminal amino acid sequence of the 59 kDa protein in starch granules from leaves, GMKLIFVGMEVGP, was homologous to, but somewhat different from, that of PvGBSSIa. A cDNA clone (designated pvgbsslb) for the leaf protein (PvGBSSIb) was isolated from kidney bean leaves by reverse transcriptase-mediated PCR (RT-PCR), 5'-RACE (rapid amplification of cDNA end), and 3'-RACE. The pre dicted amino acid sequence of mature PvGBSSIb displayed significant identity (83%) to that of pea GBSSIb. Analysis of RT-PCR products indicated that the pvgbsslb transcripts predominantly accumulate in leaves. Recombinant PvGBSSIb was purified from Escherichia coil and some enzymatic properties were investigated.

Journal

• Journal of Applied Glycoscience

  Journal of Applied Glycoscience 50 (4), 493-497, 2003

  The Japanese Society of Applied Glycoscience

Keywords

• granule-bound starch synthase Ib, kidney bean, Phaseolus vulgaris L., starch biosynthesis

Details

• CRID: 1390282681268320512
• NII Article ID: 10012469080
• NII Book ID: AN10453916
• DOI: 10.5458/jag.50.493
• COI: 1:CAS:528:DC%2BD3sXpsVantrw%3D
• ISSN: 18807291
• ISSN: 13447882
• ISSN: http://id.crossref.org/issn/13447882
• NDL BIB ID: 6761610
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6761610
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/50/4/50_4_493/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed