間葉系幹細胞, フィブリンのりおよび生体吸収性足場を用いた注入法による骨再生に関する実験的研究 Experimental study of injectable bone for regeneration using mesenchymal stem cells, fibrin glue, and a biodegradable scaffold

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著者

    • 小澤 亮太郎 OZAWA Ryotaro
    • 名古屋大学大学院医学研究科頭頸部感覚器外科学講座咀嚼障害制御学 Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
    • 山田 陽一 YAMADA Yoichi
    • 名古屋大学医学部付属病院遺伝子再生医療センター Center for Genetic and Regenerative Medicine, Nagoya University school of Medicine
    • 日比野 祥敬 HIBINO Yoshitaka
    • 名古屋大学大学院医学研究科頭頸部感覚器外科学講座咀嚼障害制御学 Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
    • 長坂 徹郎 NAGASAKA Tetsuro
    • 名古屋大学大学院医学研究科高次医用科学講座 Department of Laboratory Medicine, Nagoya University, Postgraduate School of Medicine
    • 夫 才成 FU Saisei
    • 名古屋大学大学院医学研究科頭頸部感覚器外科学講座咀嚼障害制御学 Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
    • 岡崎 恭宏 OKAZAKI Yasuhiro
    • 名古屋大学大学院医学研究科頭頸部感覚器外科学講座咀嚼障害制御学 Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
    • 畠 賢一郎 HATA Ken-ichiro
    • 名古屋大学医学部付属病院遺伝子再生医療センター Center for Genetic and Regenerative Medicine, Nagoya University school of Medicine
    • 上田 実 UEDA Minoru
    • 名古屋大学大学院医学研究科頭頸部感覚器外科学講座咀嚼障害制御学 Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine

抄録

The purpose of this study was to investigate whether a combination of fibrin glue, biodegradable scaffold (β-tricalcium phosphate;β-TCP), and mesenchymal stem cells (MSCs) would promote new bone formation and provide three-dimensional templates for bone growth at heterotopic sites in rats with plasticity. Growing MSCs and developing matrices, explanted from the rat femur, were minced and mixed with fibrin glue in a syringe. The MSCs/fibrin glue-β-TCP complex was injected into the subcutaneous space of the back of the rat. Eight weeks after implantation, macroscopic examination revealed a pearly opalescence that was firm on palpation. Histologic investigations showed newly formed bone structures in all rats treated with the MSCs/fibrin glue-β-TCP complex. No bone structures were found in the fibrin glue or β-TCP control groups. Our results demonstrate that injectable MSCs/fibrin glue-β-TCP complex can provide a three-dimensional scaffold for the successful transplantation and engraftment of osteoblasts. This technique holds promise of becoming a minimally invasive means of delivering autogenous bone to correct or repair bone defects such as those associated with osteoprotic fractures, paradontosis, and tumor resection.

収録刊行物

  • 日本口腔外科学会雑誌

    日本口腔外科学会雑誌 49(5), 317-322, 2003-05-20

    Japanese Society of Oral and Maxillofacial Surgeons

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各種コード

  • NII論文ID(NAID)
    10012629220
  • NII書誌ID(NCID)
    AN00189163
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215163
  • データ提供元
    CJP書誌  J-STAGE 
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