Enhancement of Reporter-Gene Expression by Insertions of Two Introns in Maize and Tobacco Protoplasts

この論文にアクセスする

この論文をさがす

著者

抄録

The effects of two-intron insertions on reporter-gene expression were examined in transient assays in maize and tobacco. The first introns of a rice gene for phospholipase D (PLD), a maize gene for ubiquitin and a castor bean gene for catalase were tested with a gene for β-glucuronidase (GUS) or a gene for luciferase driven by a cauliflower mosaic virus 35S promoter (35S-GUS or 35S-Luc), the promoter of the maize ubiquitin (Ubi-GUS) or the promoter of a maize pyruvate, orthophosphate dikinase (PPDK-GUS). Although the enhancing effect varied, several intron combinations synergistically enhanced the reporters. When the first and second introns of the PLD gene were inserted in tandem, the reporter was synergistically enhanced. These results imply that expression enhancement by two introns may be observed in wide combinations of promoters, introns, and structural genes and that such enhancement plays certain roles in regulation of gene expression in higher plants.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 21(1), 15-24, 2004-03-01

    日本植物細胞分子生物学会

参考文献:  38件中 1-38件 を表示

各種コード

  • NII論文ID(NAID)
    10012727045
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    6884178
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ