Molecular Cloning of a cDNA that Encodes a Spinach Cysteine Synthase-Like Protein

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In Spinacia oleacea, three isoforms of cysteine synthase (CSase, O-acetyl-serine (thiol) lyase) were identified in the cytosolic, plastidial, and mitochondorial compartments. Here, we report molecular cloning of a cDNA that encodes a CSase-like protein, designated CSaseLP. The predicted amino acid sequence of CSaseLP showed high identity (about 70%) with that of a spinach cytosolic CSase (encoded by CSaseA gene). The CSase activity of recombinant CSaseLP was barely detectable. The antibody raised against the recombinant CSaseLP protein recognized weakly a recombinant CSaseA protein. Northern blot analysis showed that CSaseLP gene was expressed mainly in root tissues, while the expression of CSaseA gene was constitutive in leaf and root tissues. These results suggest that the CSaseLP protein, though belongs to the CSase family, play a role distinct from the cytosolic CSase.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 21(1), 73-77, 2004-03-01

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10012727231
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    6884314
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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