低脂肪乳による大規模食中毒事件におけるブドウ球菌エンテロトキシンA遺伝子検出の意義 Significance of the Detection of Staphylococcal Enterotoxin A Gene in Low Fat Milk Which Caused a Serious Outbreak of Food Poisoning

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著者らは,雪印乳業食中毒事件に際し,有症者が飲んだ低脂肪乳からPCR法で迅速にブドウ球菌エンテロトキシン(SE) A遺伝子を検出したことから,本事件はSEAによるものと推定した.SEA遺伝子検出の意義を検討するため,市販低脂肪乳などからSE遺伝子の検出を試みるとともに,黄色ブドウ球菌を添加した低脂肪乳中のSEA遺伝子の検出下限を求めた結果,正常な市販低脂肪乳などからSEA遺伝子は通常検出されないことが分かった.本事件のように,菌の培養および毒素の検出が困難な食中毒事件において,PCR法を用いた迅速な原因推定は大きな意義があるものと考えられる.

In June 2000, there was a large-scale outbreak of food poisoning after consumption of Snow Brand low fat milk. In the evening of a day the incident made public, some cartons of low fat milk were brought to our laboratory for examination. Next day, we detected only staphylococcal enterotoxin (SE) A gene among SE (A∼E) genes by PCR in left-over milk samples or samples from the same lots that patients had consumed. We presumed that the outbreak was caused by the intake of SEA. We subsequently confirmed the presence of SEA in these samples.<br>To investigate the existence of SE (A∼E) genes in milk, we examined 100 samples of commercial low fat milk and milk by PCR, but none of the genes was detected. We estimated the detection limit of SEA gene in low fat milk by PCR. Four strains of SEA-producing <i>Staphylococcus aureus</i> cultures were serially diluted in low fat milk. The SEA gene was detected at levels of 5.5×10<sup>2</sup> to 1.6×10<sup>4</sup> cfu/mL of <i>S. aureus.</i> These amounts of <i>S. aureus</i> are higher than the values in raw milk reported previously. Therefore we consider that SE genes in low fat milk should usually be undetectable by our PCR.<br>This study shows that quick detection of SE genes by PCR is very helpful to analyze outbreaks, especially if no significant bacterium can be cultured.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 44(4), 186-190, 2003-08-25

    [日本食品衛生学会]

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被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10012728912
  • NII書誌ID(NCID)
    AN00117741
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00156426
  • NDL 記事登録ID
    6690868
  • NDL 雑誌分類
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL 請求記号
    Z19-364
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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