Preconcentration of Catecholamines into Liposomes with Imposed pH Gradients

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The preconcentrations of epinephrine (EP) and norepinephrine (NE) into phosphatidylcholine liposomes with imposed pH gradient across the membrane were investigated by adding the catecholamine (CA) to the external medium of liposomes. To determine the CAs entrapped into liposomes, a simple method was also developed, based on the adsorption of CAs onto alumina and chemiluminescence detection. The uptake of the CAs into the liposomes was examined over a wide ΔpH range of 0–5 units (internal pH 5.0). The maximum uptakes of EP and NE were 70% and 88%, respectively, around an external pH of 9 (ΔpH 4), where zwitterionic species of EP and NE are dominant. The final concentrations of EP and NE in internal volumes were 34- and 43-fold greater than the initial concentrations of EP and NE, respectively. The uptake of the CAs was studied by using a mathematical model, which indicates that the uptake is contributed by the molar fraction of the zwitterion species in the external volume as well as the pH gradient. The uptake of EP was found to suffer from a steric hindrance due to a methyl group substituted for the hydrogen of the amino group. This study proves the capability of the liposomes for accumulation media of CAs.

収録刊行物

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 76(5), 985-989, 2003-05-15

    The Chemical Society of Japan

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各種コード

  • NII論文ID(NAID)
    10012812214
  • NII書誌ID(NCID)
    AA00580132
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00092673
  • NDL 記事登録ID
    6532917
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-B35
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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