Direct Observation of the DNA Multimolecule Condensation with Fluorescence Microscopy

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DNA condensation plays a crucial role in DNA strand-exchange, ligation, renaturation and catenation. Thus it has drawn researchers' great attention. In this work, spermine-induced DNA condensation was studied with epifluorescence microscopy. In the presence of Ru(phen)<SUB>2</SUB>(dppx)<SUP>2+</SUP> (phen = 1,10-phenanthroline, dppx = 7,8-dimethyl-dipyridophenazine), single λ-DNA with the length of about 10 μm was observed. With the addition of 20 mmol·l<SUP>−1</SUP> spermine, there occurred DNA multimolecule condensation, resulting in the formation of a 40 μm rod-like highly compacted and ordered superhelical structure with the diameter of about 7 μm. This provides the direct evidence of DNA multimolecule condensation induced by spermine in solution.

収録刊行物

  • Chemistry letters

    Chemistry letters 32(1), 80-81, 2003-01-05

    The Chemical Society of Japan

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各種コード

  • NII論文ID(NAID)
    10012813376
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    03667022
  • データ提供元
    CJP書誌  J-STAGE 
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