Fabrication of Ultrathin, Protein-containing Films by Layer-by-Layer Assembly and Electrochemical Characterization of Hemoglobin Entrapped in the Film

DOI PDF 被引用文献2件 参考文献17件
  • Libin Shang
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing UniversityNanjing 210093, P. R . China
  • Xinjian Liu
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing UniversityNanjing 210093, P. R . China
  • Jun Zhong
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing UniversityNanjing 210093, P. R . China
  • Chunhai Fan
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing UniversityNanjing 210093, P. R . China
  • Iwao Suzuki
    Graduate School of Tohoku UniversityAramaki, Aoba-ku, Sebdai
  • Genxi Li
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing UniversityNanjing 210093, P. R . China

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<jats:title>Abstract</jats:title> <jats:p>Multilayers of hemoglobin were uniformly deposited on a pyrolytic graphite electrode surface through a layer-by-layer self-assembly approach. The well-known anionic polyelectrolyte, DNA, was used to attract cationic hemoglobin due to the strong electrostatic interaction between DNA and the protein. Interestingly, the electron transfer reactivity of hemoglobin was significantly improved in the film, which in turn facilitated the electrocatalytic reduction of nitric oxide.</jats:p>

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  • Chemistry Letters

    Chemistry Letters 32 (3), 296-297, 2003-02-25

    Oxford University Press (OUP)

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