Affinity Capillary Electrophoresis in a Poly(dimethylsiloxane)-glass Hybrid Microchip

  • Ito Toshiyuki
    Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • Inoue Akira
    Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • Sato Kae
    Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • Hosokawa Kazuo
    Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • Maeda Mizuo
    Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)

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抄録

Affinity capillary electrophoresis of DNA was conducted in a poly(dimethylsiloxane)-glass hybrid microchip with a 100 μm (width) × 130 μm (height) microchannel containing poly(N,N-dimethylacrylamide)-DNA conjugate. By this method, sample 12-mer oligonucleotide composed of c-K-ras codon 10-13 was separated from its single-base substituted mutant within 2 min.

収録刊行物

  • Chemistry Letters

    Chemistry Letters 32 (8), 688-689, 2003

    公益社団法人 日本化学会

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