Gene Diagnosis Using DNA-linked Nanoparticles

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  • ＤＮＡ担持ナノ粒子による遺伝子診断
  • DNA タンジ ナノ リュウシ ニ ヨル イデンシ シンダン

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Abstract

Highly sensitive and selective DNA detection has attracted extensive attention for its importance in clinical gene diagnosis. Recently, we developed a simple method for visually detecting single nucleotide mutation by using spontaneous aggregation of DNA-linked colloidal nanoparticles, which was prepared through the phase transition of the amphiphilic copolymer consisting of DNA as the hydrophilic part and thermo-responsive poly (N-isopropylacrylamide) as the hydrophobic part. Adding the complementary DNA into the nanoparticles dispersion, the nanoparticles aggregated rapidly by a non-crosslinking mechanism, based on the salting-out techniques. The aggregation of DNA-linked colloidal nanoparticles was induced by the duplex formation on their surfaces. The instabilization of the nanoparticles would be derived from the decrease of both electrostatic repulsion and entropic effect of the DNA strand tethered to the surfaces of nanoparticles. In contrast, the nanoparticles kept dispersing in the presence of the point-mutated DNA under the identical conditions. Moreover, we improved this method for detecting single nucleotide mutation of sample DNA longer than the DNA attached to the surfaces of the nanoparticles.

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