Identification of a Collagen Production-promoting Factor from an Extract of Royal Jelly and Its Possible Mechanism

• KOYA-MIYATA Satomi

  Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.

• OKAMOTO Iwao

  Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.

• USHIO Shimpei

  Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.

• IWAKI Kanso

  Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.

• IKEDA Masao

  Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.

• KURIMOTO Masashi

  Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.

抄録

We have previously shown that royal jelly (RJ) promoted collagen production by skin fibroblasts in the presence of ascorbic acid-2-O-alpha-glucoside (AA-2G). In this study, we purified the honeybee RJ-derived collagen production-promoting factor (HBRJ-CPF) from an alkali-solubilized fraction of RJ by C18 reverse-phase column chromatography. The elution profile by the C18 column chromatography and the molecular mass of the purified HBRJ-CPF material coincided with those of 10-hydroxy-2-decenoic acid (10H2DA). We then examined the collagen production-promoting activities of several commercially available fatty acids contained in RJ. We found that 10H2DA and 10-hydroxydecanoic acid increased the collagen production in a dose-dependent manner. Furthermore, 10H2DA induced the fibroblast cell line, NHDF, to produce transforming growth factor-β1 (TGF-β1) which is an important factor for collagen production. As expected, the collagen production-promoting activity of 10H2DA was neutralized by the anti-TGF-β1 antibody. These result suggest that HBRJ-CPF identified as 10H2DA promoted the collagen production of AA-2G-treated fibroblasts by inducing TGF-β1 production.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 68 (4), 767-773, 2004

  公益社団法人 日本農芸化学会

キーワード

• royal jelly (RJ)
• collagen production
• 10-hydroxy-2-decenoic acid (10H2DA)
• transforming growth factor-β (TGF-β1)

詳細情報

• CRID: 1390282681450421248
• NII論文ID: 10013143505
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.68.767
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 6930119
• PubMed: 15118301
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6930119
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.68.767
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可