Improved Expression of Novel Red- and Green-emitting Luciferases of<i>Phrixothrix</i>Railroad Worms in Mammalian Cells

  • NAKAJIMA Yoshihiro
    Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST)
  • KIMURA Takuma
    Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST)
  • SUZUKI Chie
    Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST) TOYO B-Net Co.
  • OHMIYA Yoshihiro
    Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST)

書誌事項

タイトル別名
  • Improved Expression of Novel Red- and Green-emitting Luciferases of Phrixothrix Railroad Worms in Mammalian Cells
  • Improved expression of novel red- and green-emitting luciferases of <italic>Phrixothrix</italic> railroad worms in mammalian cells.
  • Improved expression of novel red- and green-emitting luciferases of Phrixothrix railroad works in mammalian cells

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Luciferases are widely used for the quantitative monitoring of gene expression in a variety of organisms. We successfully expressed novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells in combination with the Kozak sequence and the CAG promoter. The characteristic properties of these luciferases indicate that they are appropriate reporter genes for the simultaneous monitoring of two gene expressions.

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