Identification of High Molecular Weight Proteins in Squid Muscle by Western Blotting Analysis and Postmortem Rheological Changes

DOI Web Site Web Site PubMed 被引用文献1件 参考文献51件
  • KASAMATSU Chinatsu
    School of Human Life and Environmental Science, Ochanomizu University Department of Nutrition and Dietetics, Kamakura Women’s University
  • KIMURA Sumiko
    Faculty of Science, Chiba University
  • KAGAWA Mieko
    Komatsu High School
  • HATAE Keiko
    School of Human Life and Environmental Science, Ochanomizu University

この論文をさがす

抄録

The high molecular weight protein connectin (also called titin) in Japanese common squid (Todarodes pacificus) mantle muscle was identified by western blotting analysis with 3B9, the mouse anti-chicken skeletal muscle connectin monoclonal antibody. Similarly to vertebrate samples, there exists connectin in invertebrate squid mantle muscle, and the amino acid sequences are assumed to resemble those present in the A band of vertebrate connectin, judging by the specificity of 3B9. Moreover, the connectin in squid muscle migrated in this study as a closely spaced doublet of α and β (titins 1 and 2). Between 5 and 7 h post-mortem, the SDS PAGE patterns of the squid sample indicated a change of the doublet bands into a single β-connectin band. Simultaneously, the rheological properties of the squid muscle changed substantially. This degradation of α-connectin into β-connectin in the muscle can explain the critical change that occurs during the post-mortem tenderization of squid muscle.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (51)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ