Reversal of Streptozotocin-induced Hyperglycemia by Transplantation of Pseudoislets Consisting of .BETA. Cells Derived from Ductal Cells

DOI Web Site PubMed 被引用文献6件 参考文献20件
  • OGATA Takeki
    Institute for Molecular & Cellular Regulation, Gunma University Third Department of Internal Medicine, National Defense Medical College
  • PARK Kee Young
    Institute for Molecular & Cellular Regulation, Gunma University
  • SENO Masaharu
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University
  • KOJIMA Itaru
    Institute for Molecular & Cellular Regulation, Gunma University

この論文をさがす

抄録

The present study was conducted in an attempt to treat streptozotocin (STZ)-induced hyperglycemia by transplanting β cells derived from pancreatic ductal cells. Ductal cells obtained from neonatal rats were cultured in vitro. Approximately 70% of the cells were converted to insulin-secreting cells by incubating with betacellulin and activin A. Differentiated cells responded to a depolarizing concentration of potassium, tolbutamide and a high concentration of glucose, and insulin secretion increased by 2.5-, 2.3- and 1.6-fold, respectively. We then prepared pseudoislets using the differentiated cells, which exhibited greatly improved glucose-responsiveness, with a high concentration of glucose inducing a 3-fold increase in insulin secretion. We transplanted these pseudoislets into the portal vein of STZ-treated nude mice. Before transplantation, the plasma glucose concentration was above 400 mg/dl, and after transplantation it was markedly reduced, the effect of which persisted for two weeks. These results indicate that STZ-induced hyperglycemia can be treated by transplanting pseudoislets consisting of β cells derived from ductal cells.<br>

収録刊行物

  • Endocrine Journal

    Endocrine Journal 51 (3), 381-386, 2004

    一般社団法人 日本内分泌学会

被引用文献 (6)*注記

もっと見る

参考文献 (20)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ