Single-Channel Recordings of Gramicidin at Agarose-Supported Bilayer Lipid Membranes Formed by the Tip-Dip and Painting Methods

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  • MATSUNO Yuka
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • OSONO Chie
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • HIRANO Ayumi
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • SUGAWARA Masao
    Department of Chemistry, College of Humanities and Sciences, Nihon University

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Agarose-supported BLMs were prepared by the tip-dip and painting methods, and single-channel recordings of gramicidin were examined for the development of an ion-channel sensor. The supported BLMs formed by the tip-dip method had an electric resistance of >1.0 × 1011 Ω and a longer lifetime as compared with unsupported ones, which enabled single-channel recordings of gramicidin. The supported BLMs formed by the painting method also enabled single-channel recordings, but the lifetime was shorter than that of unsupported planar BLMs formed by the monolayer folding method.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 20 (8), 1217-1221, 2004

    社団法人 日本分析化学会

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