皮膚から分離されたMycobacterium shinshuenseの細菌学的解析

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タイトル別名
  • MYCOBACTERIUM SHINSHUENSE ISOLATED FROM CUTANEOUS ULCER LESION OF RIGHT LOWER EXTREMITY IN A 37-YEAR-OLD WOMAN
  • ヒフ カラ ブンリ サレタ Mycobacterium shinshuense ノ サイキンガクテキ カイセキ

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[Purpose] Second clinical infection case of Myco bacterium shinshuense was presented, we tried the identifi cation of M. shinshuense that is isolated from skin.<BR>[Object] Mycobacteria species isolated from cutaneous ulcer lesion of right lower extremity in a 37-year-old woman.<BR>[Method] Identification by DNA-DNA Hybridization, 16S rRNA and rpoB method as genomic level and conventional method.<BR>[Result] It did not grow on 1% Ogawa's slant medium at both 37°C and 42°C, but grew at 28°C. It formed yellowish colonies in the dark. It was difficult to distinguish M. shinshu ense from M. ulcerans and M. marinum by DNA-DNA hybrid ization (DDH) and DNA sequencing. To identify that it is M. shinshuense, growth rate, temperature range of mycobacterial growth, light coloration reaction, biochemical and biological tests, and drug susceptibility testing were further explored. Finally it was identified as M. shinshuense based on these results.° [Consideration] For Mycobacteria species which grow 2 weeks after inoculation at 28°C, and which is identified as M. marinum by DDH method, it is necessary to identify with sequence and conventional method.

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  • 結核

    結核 79 (7), 437-441, 2004

    一般社団法人 日本結核病学会

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