Substrate-dependent Activation of Thermolysin by Salt

• ONEDA Hiroshi

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• MUTA Yuko

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• INOUYE Kuniyo

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

抄録

Salt-activation of thermolysin was examined using a positively charged fluorescent substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH₂ [MOCAc-PLGL(Dpa)AR]. Thermolysin activity increased in a biphasic exponential fashion and was 40 times higher in the presence of 4 M NaCl than in its absence. The degree of activation at x M NaCl was expressed as 4.7^x when [NaCl]_o<0.5 M and 2.3^x when [NaCl]_o>0.5 M respectively.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 68 (8), 1811-1813, 2004

  公益社団法人 日本農芸化学会

キーワード

• metalloproteinase
• thermolysin
• salt-activation
• halophilicity

詳細情報

• CRID: 1390282681452898560
• NII論文ID: 10013521230
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.68.1811
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7067126
• PubMed: 15322372
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7067126
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.68.1811
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可