アグロバクテリウム法によるムスカリの形質転換における<i>bar</i> 遺伝子と<i>hpt</i> 遺伝子の選抜効率の比較

DOI 機関リポジトリ NDLデジタルコレクション 日本農学文献記事索引 HANDLE ほか2件をすべて表示 一部だけ表示 被引用文献1件 参考文献17件

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  • Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum

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Embryogenic calli of Muscari armeniacum cv. Blue Pearl were co-cultivated with Agrobacterium tumefaciens EHA101/pBH, which harbored a binary vector that carries the phosphinothricin acetyltransferase (bar) and hygromycin phosphotransferase (hpt) genes. The calli were then transferred onto the selection media containing either 4 mg · liter−1 bialaphos or 75 mg · liter−1 hygromycin. Four to five weeks after transfer to the selection media, both bialaphos-resistant (Biar) and hygromycin-resistance (Hygr) cell clusters were produced. Over 90% of callus lines selected on a bialaphos-containing medium were verified to be transgenic by PCR analysis; this selection efficiency is comparable to that based on the hpt gene. Leaf segments of plantlets regenerated from the transgenic Biar callus lines showed resistance to 4 mg · liter−1 of bialaphos, indicating that the bar gene is useful not only as a selectable marker but also as a producer of herbicide-resistant transgenic plants of M. armeniacum.

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  • 園芸学会雑誌

    園芸学会雑誌 74 (1), 60-62, 2005

    一般社団法人　園芸学会

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