アフリカツメガエル初期胚を用いた試験管内における眼の形成と移植実験系の確立

• 後原 綾子

  東京大学大学院総合文化研究科生命環境科学系浅島研究室

• 浅島 誠

  東京大学大学院総合文化研究科生命環境科学系浅島研究室 International Cooperative Research Project (ICORP), Japan Science and Technology Corporation (JST)

書誌事項

タイトル別名

• <i>In vitro</i> induction and transplantation of eye during early Xenopus development

抄録

We established a novel in vitro system to induce eye at high frequency using Xenopus early gastrulae. The eye formed in vitro is morphologically similar to the normal eye. Immunostaining showed that the eye induced in vitro had mature optic tissues. The cell lineage tracing revealed that eyes formed in vitro were derived from the animal cap cells. For functional analysis, we tried transplantation of eye induced in vitro. When eye induced in vitro was transplanted into stage 33 eyeless tadpole, the grafted eye rooted to the host tadpole and optic nerve was regenerated. Dil stanining of the optic nerve showed that regenerated optic nerve reached the tectum of the host brain. The grafted eye was retained after metamorphosis. The resultant juvenile frogs could perceive brightness using the grafted eye and control their skin color, suggesting that the eye formed in vitro could function normally.

収録刊行物

• 炎症・再生

  炎症・再生 25 (2), 107-112, 2005

  一般社団法人 日本炎症・再生医学会

キーワード

• eye
• organ engineering
• regeneration
• transplantation

詳細情報

• CRID: 1390001205178969856
• NII論文ID: 10015450027
• NII書誌ID: AA11508953
• DOI: 10.2492/jsir.25.107
• ISSN: 18805795
• ISSN: 13468022
• Web Site: https://www.jstage.jst.go.jp/article/jsir/25/2/25_2_107/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005149121
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可