Phenotypic and Genetic Characterization of the Three Strains of a Marine Red Alga Porphyra yezoensis (Bangiales, Rhodophyta)
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- PARK Eun-Jeong
- Graduate School of Fisheries Sciences, Hokkaido University
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- TAKAHASHI Megumu
- Graduate School of Fisheries Sciences, Hokkaido University
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- KITADE Yukihiro
- Graduate School of Fisheries Sciences, Hokkaido University JSPS Research Fellow, Japan Society for the Promotion of Science
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- YASUI Hajime
- Graduate School of Fisheries Sciences, Hokkaido University
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- SAGA Naotsune
- Graduate School of Fisheries Sciences, Hokkaido University
Bibliographic Information
- Other Title
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- 海産紅藻スサビノリ (紅色植物門・ウシケノリ目) 3株の表現型と遺伝的特性
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Abstract
Polymorphisms of the three strains TU-1 (wild type), TU-2 (green type) and TUH-25 (red type) inPorphyra yezoensis were investigated using cultured monospores and cleaved amplified polymorphic sequence (CAPS) analysis of gametophytes in each strain. Comparison of early developmental characters of monospores, such as the size, cell number at the first longitudinal cell division, and change of the cell number of germlings over time, indicated that these characters are useful for phenotypic classification of the three strains. In the CAPS analysis using 19 PCR primer pairs and 22 restriction endonucleases, no length polymorphism in the restriction fragments was detected among the three strains of P. yezoensis examined. These results suggest that a distinctive phenotypic character in early developmental stage of monospore is not always associated with a genetic polymorphism. Therefore, the three strains may be too close genetically, and not applicable to a linkage analysis of P. yezoensis using CAPS markers employed herein.
Journal
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- Aquaculture Science
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Aquaculture Science 53 (1), 31-40, 2005
Japanese Society for Aquaculture Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001204718094720
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- NII Article ID
- 10015597151
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- NII Book ID
- AN00124667
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- ISSN
- 21850194
- 03714217
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- NDL BIB ID
- 7294945
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed