Repeated Stress Suppresses Interferon-.GAMMA. Production by Murine Intestinal Intraepithelial Lymphocytes
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- Zhang Xiumin
- Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medicine
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- Okutsu Mitsuharu
- Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medicine
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- Kanemi Osamu
- Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medicine
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- Gametchu Bahiru
- Department of Pediatrics, Medical College of Wisconsin
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- Nagatomi Ryoichi
- Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medicine
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抄録
Intestinal intraepithelial lymphocytes (IEL), one of the major effector components in the mucosal immune system, are phenotypically and functionally distinct from thymic and peripheral T cells. To investigate the effect of repeated stress on the number and function of IEL, we exposed male C3H/HeN mice to mild electric foot shock for 30 min/day for 5 consecutive days. Immediately after the final foot shock stress, the blood, spleen, thymus and small intestine of each of the mice were obtained. As a functional measure, we evaluated interferon (IFN)- γ production by IEL, since IFN-γ is a key immunomodulating cytokine in mucosal immune responses. Serum corticosterone level was elevated immediately after foot shock stress. There were no significant changes in the number of whole IEL and CD3+ IEL subsets after the stress. In contrast, the stress led to a significant decrease in the total number of thymocytes, particularly the reduction in the number of CD4+CD8+ thymocytes. Thymocytes expressed the highest level of intracellular glucocorticoid receptor (GR), followed by splenocytes and IEL. The foot shock stress induced a marked suppression of IFN-γ production by IEL, when stimulated with immobilized anti-CD3 monoclonal antibody. Furthermore, corticosterone suppressed the IFN-γ production by cultured IEL, which was prevented by Mifepristone (RU486), a GR antagonist. In summary, repeated foot shock stress did not alter the numbers of IEL and CD3+ IEL subsets, but suppressed IFN-γ production by IEL, which was probably mediated by the elevated corticosterone. We therefore propose that stress influences host defense by suppressing the production of IFN-γ in IEL.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 206 (3), 203-212, 2005
東北ジャーナル刊行会
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詳細情報 詳細情報について
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- CRID
- 1390282679216892672
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- NII論文ID
- 10016132390
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- NII書誌ID
- AA00863920
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- ISSN
- 13493329
- 00408727
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- PubMed
- 15942146
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可