パンジー花色の花弁フラボノイド組成のHPLCによる系統評価(育種・遺伝資源)  [in Japanese] HPLC Evaluation of Anthocyanins and Flavonols in Relation to the Flower Color of Pansies (Viola×wittrockiana Gams)  [in Japanese]

パンジーにおけるHPLCを用いたフラボノイド分析を行った.その結果, それぞれ6種類のアントシアニンおよびフラボノール類が検出された.そのうち, 4種類のアントシアニンはDp3RG, Cy3RG, Dp3pCRG5G(ナスニン)およびCy3pCRG5Gであり, また1種類のフラボノール類はQu3RG(ルチン)であった.黄色および白色系統においてアントシアニンは検出されなかった.青色系統はナスニンおよび6種類全てのフラボノール類が主要色素として存在し, 系統間でその含量の違いが認められたことから, コピグメントによる花色幅の拡大の可能性が示唆された.赤色系統はアントシアニンのCy3RGおよびルチンを含む3種類のフラボノール類が主要色素であり, また赤紫系統では特異的なアントシアニン(Cy3pCRG5G)が多く含まれ, 赤色系統および赤紫系統はアントシアニンの違いにより花色が異なることが示唆された.これらのことから, パンジーの新規花色品種育種おけるHPLC分析の有効性が示された.

Anthocyanins and flavonols were analyzed by HPLC using inbread lines and varieties of Pansy (Viola×wittrockiana). Each of the six main anthocyanins and flavonols were detected by HPLC. Four anthocyanins were identified by co-chromatography to be Dp3RG, Cy3RG, Dp3pCRG5G (nasunin) and Cy3pCRG5G. One of the flavonols was identified by co-chromatography to be Qu3RG (rutin). Yellow and white lines did not contain anthocyanin. Nasunin, rutin and five flavonols were detected in bluish color. There were differences in the amounts of major flavonols between lines. This result indicated the possibility of anthocyanin-flavonol copigmentation in variations of bluish color. Cyanidin-3-ramnosylglucoside, rutin and five flavonols were detected in reddish flowers. Major flavonols were No.3, No.4, and rutin in red lines. Another anthocyanin (Cy3pCRG5G) was detected in reddish-purple line. Differences in anthocyanin influenced the color variation of reddish colors. These findings indicated the importance of HPLC analysis in pansy breeding.