Biochemical Characterization of TT1383 from Thermus thermophilus Identifies a Novel dNTP Triphosphohydrolase Activity Stimulated by dATP and dTTP
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- Kondo Naoyuki
- Department of Biology, Graduate School of Science, Osaka University
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- Kuramitsu Seiki
- RIKEN Harima Institute at SPring-8
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- Masui Ryoji
- RIKEN Harima Institute at SPring-8
書誌事項
- タイトル別名
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- Biochemical Characterization of TT 1383 from <i>Thermus thermophilus</i> Identifies a Novel dNTP Triphosphohydrolase Activity Stimulated by dATP and dTTP
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The HD domain motif is found in a superfamily of proteins in bacteria, archaea and eukaryotes. A few of these proteins are known to have metal-dependant phosphohy-drolase activity, but the others are functionally unknown. Here we have character-ized an HD domain-containing protein, TT1383, from Thermus thermophilus HB 8. This protein has sequence similarity to Escherichia coli dGTP triphosphohydrolase, however, no dGTP hydrolytic activity was detected. The hydrolytic activity of the protein was determined in the presence of more than two kinds of deoxyribonucleo-side triphosphates (dNTPs), which were hydrolyzed to their respective deoxyribonu-cleosides and triphosphates, and was found to be strictly specific for dNTPs in the fol-lowing order of relative activity: dCTP>dGTP>dTTP>dATP. Interestingly, this dNTP triphosphohydrolase (dNTPase) activity requires the presence of dATP or dTTP in the dNTP mixture. dADP, dTDP, dAMP, and dTMP, which themselves were not hydrolyzed, were nonetheless able to stimulate the hydrolysis of dCTP. These results suggest the existence of binding sites specific for dATP and dTTP as positive modulators, distinct from the dNTPase catalytic site. This is, to our knowledge, the first report of a non-specific dNTPase that is activated by dNTP itself.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 136 (2), 221-231, 2004
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204966056192
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- NII論文ID
- 130003418167
- 10016201562
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD2cXpslKrsb0%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 7068390
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- PubMed
- 15496593
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可