Streptococcus rattus に対する単クロン抗体 : 細胞壁抗原決定基の免疫化学的性質とその局在

• 五嶋 淑雄

  九州歯科大学口腔細菌学講座

書誌事項

タイトル別名

• Monoclonal Antibodies Specific for Streptococcus rattus : Specificity and Electron Microscopic Observation of Cell Wall Antigenic Determinants

抄録

Several hybridomas producing monoclonal antibodies specific for Streptococcus rattus (S. rattus) were prepared by fusing myeloma cells with the spleen cells immunised with S. rattus strain FA1. One of the hybridomas was injected into BALB/c mice to produce ascitic fluids from which monoclonal antibody 2-27 (MAb 2-27, IgM) was partially purified after precipitation with ammonium sulphate. Reactivity of MAb 2-27 was examined in EIA against various strains of mutans streptococci as well as other streptococcal species. It was shown that MAb 2-27 reacted only with S. rattus strains and not with any other strain of the mutans group nor with other streptococcal strains tested. Galactosamine, glucosamine and N-acetylgalactosamine strongly inhibited the reaction of the monoclonal antibody with whole cells of S. rattus and a polysaccharide cell wall antigen purified from S. rattus strains FA1 and BHT. Immunoelectron microscopy was conducted to demonstrate distribution and localization of the specific antigen on S. rattus cells by use of MAb 2-27 plus gold-conjugated antibody. It was shown that the antigen determinant was irregularly localized on thread-like projections on the surface of the cell wall.

収録刊行物

• 九州歯科学会雑誌

  九州歯科学会雑誌 50 (2), 415-431, 1996

  九州歯科学会

キーワード

• Monoclonal antibodies
• Antigen dterminants
• Hybridoma
• Hapten
• S. ratus

詳細情報

• CRID: 1390282681322240768
• NII論文ID: 110003005373
• NII書誌ID: AN00054335
• DOI: 10.2504/kds.50.415
• ISSN: 18808719
• ISSN: 03686833
• Web Site: https://www.jstage.jst.go.jp/article/kds/50/2/50_KJ00003277321/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996210048
• 本文言語コード: ja
• データソース種別:
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可