近位主導管結紮後のラット顎下腺および舌下腺における神経組織の免疫組織学的, 超微構造的研究

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  • An Immunohistological and Ultrastructural Study on Nerves in the Rat Submandibular and Sublingual Glands after Proximal Duct Ligation

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The purpose of this study is to examine the influence of intraglandular nerves during the process of regeneration in the glandular parenchyma, in rat submandibular and sublingual glands. Forty-four adult Wistar rats were used for this study and examined by immunohistochemical and electron-microscopic techniques, from 6 hours to 60 days after proximal duct ligation. The experiments produced the following results : 1. The wet weight of submandibular and sublingual glands, rapidly increased and reached a peak a day after ligation. Then it gradually decreased. 2. Within 3 days after ligation, both intraglandular nerves and glandular parenchyma revealed severe atrophy or destruction. Both the interlobular and the periglandular connective tissue of glands, were severely edematous. All of the nerve fibers and the ganglion cells showed negative NFPstaining from 12 hours to 3 days after ligation. At 5 days after ligation, the regenerative duct epithelium appeared and formed duct-like structure, therafter, the atrophic or destructive region was gradually replaced by granulation tissue and the duct-like structure untill 14 days after ligation. At 21 days after ligation, some acinar cells appeared among these duct-like structures. At 60 days after ligation, histological findings of the gland parencyma were similar to that of control gland, but no granular convoluted tubules were observed in the new lobes. Some of the nerve fibers and ganglion cells in the interlobular region showed positive NFPstaining from 5 days to 60 days after ligation. The NFP-positive nerve fibers, which appeared around duct-like structure at 7 days, were close to the acini at 60 days after ligation. 3. On electron microscopy, degeneration of both nerve fibers and ganglion cells on the hilum were recognized within 3 days after ligation. The cytoplasm of Schwann cell containing with degenerative axons was extremely destroyed. Conversely, new Schwann cells appeared on the endoneurium of nerve bundle at 3 days after ligation, and therafter, they increased in number. Many regenerative axons were observed around the new Schwann cell. These axons and new Schwann cells were seen through the same common basement membrane. Many of sprouted axons ramified into individual fibers at 30 days. From 5 days to 30 days after duct ligation, some of ganglion cells recovered structurely. 4. Nerve fibers and ganglion cells, which have regenerated or recovered, were less than that of the control. From these results, it is concluded that (1) the time of rearrangement of nerves in rat submandibular and sublingual glands may be 21 days after proximal duct ligation, and that (2) intraglandular nerves may play an important role in the process of regeneration of the gland parenchymal cells after proximal duct ligation.

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