Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger : Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants(Pharmacognosy) MINO Yoshiki YUKITA Mayumi HIRATSUKA Nobuhiro WARIISHI Hiroyuki 公益社団法人日本薬学会 Biological & pharmaceutical bulletin 09186158 28 8 1535 1538 2005-08-01 The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1-8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants (Scopolia japonica, Datura stramonium, D. metel, and D. arborea), and only 1 or 4 differences compared to S. japonica and D. arborea. In contrast, 9-23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically. 2005-08-01 10016667413 AA10885497 7372914 ENG CJP CJPref NDL NII-ELS J-STAGE 3 17 1 Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger : Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants(Pharmacognosy) MINO Yoshiki YUKITA Mayumi HIRATSUKA Nobuhiro WARIISHI Hiroyuki The Pharmaceutical Society of Japan Biological & pharmaceutical bulletin The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1-8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants (Scopolia japonica, Datura stramonium, D. metel, and D. arborea), and only 1 or 4 differences compared to S. japonica and D. arborea. In contrast, 9-23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically. MINO Yoshiki Department of Environment Analysis, Osaka University of Pharmaceutical Sciences YUKITA Mayumi Department of Environment Analysis, Osaka University of Pharmaceutical Sciences HIRATSUKA Nobuhiro Department of Forest and Forest Products Sciences, Kyushu University WARIISHI Hiroyuki Department of Forest and Forest Products Sciences, Kyushu University