Inhibitory Effects of Trientine, a Copper-Chelating Agent, on Induction of DNA Strand Breaks in Kidney Cells of Long-Evans Cinnamon (LEC) Rats

• HAYASHI Masanobu

  Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University

• MIYANE Kazuhiro

  Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University

• SENOU Misato

  Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University

• ENDOH Daiji

  Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University

• HIGUCHI Hidetoshi

  Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

• NAGAHATA Hajime

  Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

• NAKAYAMA Kenji

  Hokkaido Institute of Public Health

• KON Yasuhiro

  Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University

• OKUI Toyo

  Hokkaido Institute of Public Health

抄録

The effects of treatment with trientine, a specific copper-chelating agent, on the accumulation of copper and induction of DNA strand breaks were investigated in Long-Evans Cinnamon (LEC) rats, an animal model for human Wilson's disease. Copper accumulated in the kidneys of LEC rats in an age-dependent manner from 12 to 18 weeks of age. When LEC rats were treated with trientine from 10 weeks of age, renal copper contents did not increase and were maintained at the same levels as those in 4-week-old LEC rats. Estimation of the amounts of DNA single-strand breaks (SSBs) by comet assay showed that SSBs of DNA were induced in a substantial population of LEC rat renal cortex cells around 12 weeks of age and that the amounts of SSBs increased in an age-dependent manner from 12 to 18 weeks of age. When LEC rats were treated with trientine from 10 weeks of age, the observed number of cells with DNA damage decreased, suggesting that induction of SSBs of DNA was inhibited and/or SSBs were repaired during the period of treatment with trientine. The results show that SSBs of DNA in LEC rat kidney cells are induced prior to occurrence of clinical signs of hepatic injury and that treatment of LEC rats with trientine decreases the number of DNA strand breaks.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 54 (5), 403-412, 2005

  公益社団法人 日本実験動物学会

キーワード

• Copper
• DNA strand breaks
• kidney cells
• LEC rat
• trientine

詳細情報

• CRID: 1390282680023170816
• NII論文ID: 10016785453
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.54.403
• COI: 1:CAS:528:DC%2BD2MXhtleks7jN
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 7681140
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7681140
• Web Site: http://www.jstage.jst.go.jp/article/expanim/54/5/54_5_403/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006309461
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可