Flow Cytometric Analysis of Gene Function
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- Matsuzaki Masaharu
- NIPPON Becton Dickinson Company, Ltd. Bioscience Laboratories
Bibliographic Information
- Other Title
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- FACSによる遺伝子機能解析
- -蛍光タンパク質を用いたHTP siRNAスクリーニング法-
- -HTP siRNA screening assay by using Fluorescent Proteins-
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Abstract
<p>Gene silencing is widely used to clarify the gene function. Although RNA interference (RNAi) can expect a handy and large effect for gene silencing, the development of the screening system of a target gene is important. Green fluorescent proteins (GFPs) and Reef Coral fluorescent proteins (RCFPs) are well known that can make stable fluoresce without any co-factors in almost all cells. Both of the function of the target gene and the fluorescent of GFPs/RCFPs are most probably kept, even if a lot of genes are made as a fusion protein with these fluorescent proteins. When GFP or RCFP is connected with C-terminal of a target protein, and if a target protein receives the resolution by RNAi, the fluorescence of GFP or RCFP in the downstream of a target protein cannot be translated to a protein and fluorescence disappears. We developed the high throughput screening system for RNAi. Fluorescence-Activated Cell Sorter (FACS) measurement of the fluorescence of 293 cells after the expression vector of a target gene and RNAi vector were co-transfected to 293 cells, a handy screening of RNAi can be established.</p>
Journal
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- Cytometry Research
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Cytometry Research 15 (1), 15-19, 2005
Japan Cytometry Society
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Keywords
Details 詳細情報について
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- CRID
- 1390282679448971904
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- NII Article ID
- 10016787719
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- NII Book ID
- AN10356261
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- ISSN
- 24240664
- 09166920
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed