Differential Effects of Buckwheat and Kudingcha Extract on Neuronal Damage in Cultured Hippocampal Neurons and Spatial Memory Impairment Induced by Scopolamine in an Eight-Arm Radial Maze

• Pu Fengling

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

• Mishima Kenichi

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

• Irie Keiichi

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

• Egashira Nobuaki

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

• Ishibashi Daisuke

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

• Matsumoto Yoshiaki

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

• Ikeda Tomoaki

  Department of Obstetrics and Gynecology, Miyazaki Medical College, University of Miyazaki

• Iwasaki Katsunori

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

• Fujii Hajime

  Amino Up Chemical Co., Ltd.

• Kosuna Kenichi

  Amino Up Chemical Co., Ltd.

• Fujiwara Michihiro

  Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

抄録

We have reported the neuroprotection provided by an extract of buckwheat (BWE, Fagopyrum esculentum Moench) on neuronal damage in the repeated cerebral ischemia model and demonstrated that BWE inhibited the excess glutamate release induced by repeated cerebral ischemia, suggesting that BWE had a free radical-scavenging in addition to anti-glutamate action. In the present study, we studied the neuroprotective effects of Kudingcha extract (KDE, Ligustrum purpurascens Y. C.) on scavenging by the 2,2-diphenyl-1-picylhydrazyl (DPPH) radical in primary cultured hippocampal neurons, and on spatial memory impairment induced by scopolamine in an eight-arm radial maze in comparison with BWE. The effects of KDE (0.01-1 mg/ml) were more potent than those of BWE (0.01-1 mg/ml) in scavenging the DPPH radical (1 mM). KDE (100 μg/ml) prevented the cell damage induced by glutamate (300 μM) or kainate (1 mM), which was more potent than BWE (100 μg/ml), but BWE suppressed the cellular damage induced by β-amyloid_(25-35) (20 μM) more potently than KDE (100 μg/ml). BWE (600 mg/kg), but not KDE, significantly suppressed the increase in errors induced by scopolamine (0.5 mg/kg i.p.) in the eight-arm radial maze. The results suggest that BWE may protect against cholinergic dysfunction and that KDE protects more effectively against glutaminergic dysfunction. <br>

収録刊行物

• ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ

  ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ 51 (6), 636-644, 2005

  公益社団法人 日本薬学会

キーワード

• buckwheat extract
• Kudingcha extract
• 2,2-diphenyl-1-picylhydrazyl radical
• primary cultured hippocampal neuron
• scopolamine

詳細情報

• CRID: 1390001204497397504
• NII論文ID: 10016881488
• NII書誌ID: AA11316464
• DOI: 10.1248/jhs.51.636
• ISSN: 13475207
• ISSN: 13449702
• NDL書誌ID: 7719864
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7719864
• Web Site: https://www.jstage.jst.go.jp/article/jhs/51/6/51_6_636/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006294963
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可