ハムスター卵の成熟ならびに受精過程におけるミトコンドリアの分布変化
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- Suzuki Hiroyuki
- Faculty of Agriculture and Life Science, Hirosaki University
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- Satoh Manabu
- Faculty of Agriculture and Life Science, Hirosaki University
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- Toyokawa Koji
- Faculty of Agriculture and Life Science, Hirosaki University
書誌事項
- タイトル別名
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- Changes in Distribution of Active Mitochondria during Oocyte Maturation and Fertilization in the Hamster
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The localization and activity of mitochondria in hamster oocytes during maturation and fertilization were monitored by using three different fluorescent probes: rhodamine 123 (Rh123), MitoTracker Red (MT-Red) and 3,3'-dihexyloxacarbocyanine iodide (DiOC6(3)). Oocytes aged in vivo were also examined. The germinal vesicle (GV) oocytes exhibited a range of mitochondrial organizations, from a uniform distribution to a more cortical restriction. Metaphase I (MI) and MII oocytes included numerous mitochondria, among which the fluorescence intensity increased two-fold over that of GV oocytes. About half (54.5%) of the MII oocytes exhibited a mitochondria free zone at the cortex. An approximately 10% decline in the density of mitochondria was observed in the oocytes aged 10 h post-ovulation, despite no significant changes. After fertilization, mitochondria became progressively aggregated and localized in the perinuclear region of all eggs examined. These comparative studies show that the signals for Rh123 and MT-Red were virtually coincident, but that for DiOC6(3) displayed wider areas of bright organizations regardless of the very low concentration used in this study (5 ng/ml). In addition, transition from a relatively homogeneous distribution of active mitochondria to perinuclear clustering may reflect energy production and utilization during oocyte maturation and fertilization in hamsters, as reported in other species.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 22 (3), 163-169, 2005
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390282679315286528
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- NII論文ID
- 10016956213
- 130004436698
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL書誌ID
- 7772613
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 使用不可