Histological evidence of the altered distribution of osteocytes and bone matrix synthesis in klotho-deficient mice

• Suzuki Hironobu

  Division of Anatomy and Cell Biology of the Hard Tissue, Niigata University Graduate School of Medical and Dental Sciences

• Amizuka Norio

  Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences Center for Transdisciplinary Research, Niigata University

• Oda Kimimitsu

  Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences Center for Transdisciplinary Research, Niigata University

• Li Minqi

  Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences Center for Transdisciplinary Research, Niigata University

• Yoshie Hiromasa

  Division of Periodontology, Niigata University Graduate School of Medical and Dental Sciences

• Ohshima Hayato

  Division of Anatomy and Cell Biology of the Hard Tissue, Niigata University Graduate School of Medical and Dental Sciences

• Noda Masaki

  Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University

• Maeda Takeyasu

  Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences Center for Transdisciplinary Research, Niigata University

抄録

Mice homozygous for klotho gene deletion are well established aging models as they mimic certain aspects of human senescence e.g. osteoporosis. Induced senescence may affect cellular functions and alter the histological properties of the extracellular matrices. The present study examined the histological and ultrastructural features of osteocytes and the surrounding bone matrix in klotho-deficient mice. As expected, osteoblasts showed a flattened shape with a weak immunoreactivity for alkaline phosphatase, and the bone matrix contained many empty osteocytic lacunae. The walls of both normal and empty lacunae were intensely immunopositive for osteopontin and dentin matrix protein-1, but featured an inconsistent immunoreactivity for osteocalcin and type I collagen. Not surprisingly, TUNEL-positivity, indicative of apoptosis, was found in many osteoblasts, osteocytes, and bone marrow cells of the klotho-deficient mice. In transmission electron microscopy, an amorphous matrix containing non-collagenous organic materials was recognizable around osteoblasts and in the osteocytic lacunae. Some osteoblasts on the bone surface featured these amorphous materials in vacuoles associated with their trans-Golgi network, indicating that, under klotho-deficient conditions, they synthesize and secrete the non-collagenous structures. Some osteocytes displayed pyknosis or degenerative traits. Thus, our findings provide histological evidence that klotho gene deletion influences the spatial distribution of osteocytes and the synthesis of bone matrix proteins in addition to the accelerated aging of bone cells.

収録刊行物

• Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ

  Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ 68 (5), 371-381, 2005

  国際組織細胞学会

キーワード

• Histology

詳細情報

• CRID: 1390282681387171840
• NII論文ID: 130004462657
• NII論文ID: 10017244382
• NII書誌ID: AA1068990X
• DOI: 10.1679/aohc.68.371
• ISSN: 13491717
• ISSN: 09149465
• Web Site: http://www.jstage.jst.go.jp/article/aohc/68/5/68_5_371/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006295934
• 本文言語コード: en
• データソース種別:
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  • CiNii Articles
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