Differential Fluorescent Banding and Isozyme Assay of Hibiscus cannabinus L. and H. sabdariffa L. (Malvaceae)
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- Hiron Naznin
- Department of Botany, University of Dhaka
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- Alam Nazmul
- Department of Botany, Jahangirnagar University
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- Ahmed Fakhruddin Ali
- Department of Botany, Jahangirnagar University
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- Begum Rabeya
- Department of Botany, University of Dhaka
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- Alam Sheikh Shamimul
- Department of Botany, University of Dhaka
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Hibiscus cannabinus var. HC-2 (2n=2x=36) and H. sabdariffa var. HS-24 (2n=4x=72) were cytogenetically and biochemically studied to elucidate their genomic homology. Both the species possessed only metacentric chromosomes. The range of chromosome length of these 2 species was 1.43–3.80 μm. Both the species showed a gradual decrease in chromosome length. Six CMA-positive bands were found in both the species. Two entirely CMA-positive banded chromosomes were found in both the species. Twenty six DAPI-positive bands were found in H. cannabinus var. HC-2. In H. sabdariffa var. HS-24, 14 DAPI- positive bands appeared. These bands were distributed in different location of respective chromosomes. Two chromosomes of H. cannabinus var. HC-2 and 8 chromosomes of H. sabdariffa var. HS-24 were entirely banded with DAPI. The entirely CMA- and DAPI-banded chromosomes were unique and could be used as marker. In H. cannabinus var. HC-2, a pair of satellites was found in orcein and CMA-staining. No satellite was found in DAPI-staining indicates its GC-rich nature. The activity of acid phosphatase and peroxidase was found similar in both the species. However, the 2 species showed different activities for esterase. The karyotypes (conventional and fluorescent) and electrophoresis pattern (acid phosphatase and peroxidase) indicates that either out of 2 different genomes in H. sabdariffa var. HS-24, 1 is very close to that of H. cannabinus var. HC-2 or H. sabdariffa var. HS-24 and H. cannabinus var. HC-2 may have derived their part of genomes from a common ancestor.
収録刊行物
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- CYTOLOGIA
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CYTOLOGIA 71 (2), 175-180, 2006
公益財団法人 日本メンデル協会
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詳細情報 詳細情報について
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- CRID
- 1390282679082685184
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- NII論文ID
- 10017390599
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- NII書誌ID
- AA00622764
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- ISSN
- 13487019
- 00114545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 使用不可