象牙質接着強さにおけるコラゲナーゼの影響 Effect of collagenase on resin-dentin bond strengths

この論文にアクセスする

この論文をさがす

著者

    • 伊藤 修一 ITO Shuichi
    • 北海道医療大学歯学部歯科保存学第二講座 Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido
    • 斎藤 隆史 SAITO Takashi
    • 北海道医療大学歯学部歯科保存学第二講座 Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido

抄録

本研究の目的は, 象牙質接着システムにおけるコラゲナーゼの影響を微小引張り接着強さの測定を用いて調べることである.試料は50本のヒト抜去大臼歯を用いた.ワンステップ接着システムとしてiBond (iB, Heraeus Kulzer), トータルエッチングシステムとしてOne Step Plus (OSP, Bisco) を用い, メーカーの指示どおりに象牙質に塗布した.OSPは, エッチングを15秒あるいは60秒処理した後, ボンディング材を塗布した.24時間後, 試料断面が0.9mm<SUP>2</SUP>になるように調製し, 1, 14, 30日間0.006%のコラゲナーゼ溶液, またはリン酸緩衝溶液 (コントロール溶液)(37QC) に浸漬し, 微小引張り接着強さの測定を行った.OSPのコラゲナーゼ溶液浸漬30日後の接着強さは, コントロール溶液浸漬と比較して, 15秒エッチングでは接着強さに有意な差は認められないものの, 60秒エッチングでは有意に低くなった.iBにおいては, コントロール溶液浸漬群とコラゲナーゼ浸漬群の接着強さに有意差は認められなかった.<BR>本研究の結果から, コラゲナーゼ溶液浸漬は, オーバーエッチングしたOSPの接着強さに影響を与えることが明らかになった.

The purpose of this work was to determine an influence of resin-dentin interfaces when specimens was incubated in Clostridium collagenase using microtensile bond test. Superficial occlusal dentin of fifty extracted human molars was used. An all-in-one adhesive, iBond (iB) and a total-etch adhesive One Step Plus (OSP) were applied to dentin surfaces according to the manufacturer's instructions. OSP was applied to dentin that was acid etched for15vs 60 sec etching. AP-X composite was used to create resin composite buildups for both adhesives. After24hours storage in37°C water, the teeth were sectioned to produce beams with0.9mm<SUP>2</SUP>cross-sectional areas for microtensile testing. The beams bonded specimens were stored in0.006% Clostridium collagenase solution or control buffer for1, 14or30days. Microtensile bond test was performed, and data were evaluated by two-way ANOVA (material vs. etching time) followed by multiple comparisons at α=0.05. For the OSP adhesive system, the tensile bond strength of specimens acid-etched for15sec showed no significant differences until30days periods. However, the OSP bond strengths of specimens acid-etched for60sec showed significantly decrease when they were stored for30days in collagenase solution, but not in control solution. In the specimens bonded with iBond, there was no decrease in bond strength in either the control or the collagenase groups over30days. Clostridium collagenase had no effect on resin-dentin bond strengths with iBond treated specimens, and only lowered bond strengths in One Step Plus bonded specimens if they were over-etched.

収録刊行物

  • 接着歯学 = Adhesive dentistry  

    接着歯学 = Adhesive dentistry 24(2), 75-82, 2006-08-15 

    Japan Society for Adhesive Dentistry

参考文献:  28件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

ページトップへ