Effect of collagenase on resin-dentin bond strengths

DOI 28 References
  • ITO Shuichi
    Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido
  • SAITO Takashi
    Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido

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Other Title
  • 象牙質接着強さにおけるコラゲナーゼの影響

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Abstract

The purpose of this work was to determine an influence of resin-dentin interfaces when specimens was incubated in Clostridium collagenase using microtensile bond test. Superficial occlusal dentin of fifty extracted human molars was used. An all-in-one adhesive, iBond (iB) and a total-etch adhesive One Step Plus (OSP) were applied to dentin surfaces according to the manufacturer's instructions. OSP was applied to dentin that was acid etched for15vs 60 sec etching. AP-X composite was used to create resin composite buildups for both adhesives. After24hours storage in37°C water, the teeth were sectioned to produce beams with0.9mm2cross-sectional areas for microtensile testing. The beams bonded specimens were stored in0.006% Clostridium collagenase solution or control buffer for1, 14or30days. Microtensile bond test was performed, and data were evaluated by two-way ANOVA (material vs. etching time) followed by multiple comparisons at α=0.05. For the OSP adhesive system, the tensile bond strength of specimens acid-etched for15sec showed no significant differences until30days periods. However, the OSP bond strengths of specimens acid-etched for60sec showed significantly decrease when they were stored for30days in collagenase solution, but not in control solution. In the specimens bonded with iBond, there was no decrease in bond strength in either the control or the collagenase groups over30days. Clostridium collagenase had no effect on resin-dentin bond strengths with iBond treated specimens, and only lowered bond strengths in One Step Plus bonded specimens if they were over-etched.

Journal

  • Adhesive Dentistry

    Adhesive Dentistry 24 (2), 75-82, 2006

    Japan Society for Adhesive Dentistry

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