ハニカム構造フィルム上におけるフィブロネクチンの吸着構造と細胞接着 Relationship between Adsorbed Fibronectin and Cell Adhesion on a Honeycomb-patterned Film

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著者

    • 新井 景子 ARAI Keiko
    • 理学研究所フロンティア研究システム時空間機能材料研究グループ Spatio-Temporal Function Materials Research Group, Frontier Research System, RIKEN Institute
    • 高山 あい子 TAKAYAMA Aiko
    • 独立行政法人科学技術振興機構戦略的創造研究推進事業 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
    • 山下 慈京子 YAMASHITA Shigeko
    • 独立行政法人科学技術振興機構戦略的創造研究推進事業 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
    • 下村 政嗣 SHIMOMURA Masatsugu
    • 独立行政法人科学技術振興機構戦略的創造研究推進事業 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

抄録

Patterned surface topographies play vital roles in cellular response such as adhesion, proliferation, and differentiation. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned porous films ("honeycomb film") of poly (ε-caprolactone) (PCL) incubated in a Fn phosphate-buffered saline (PBS) solution by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells respond to a honeycomb film, focal adhesion of porcine aortic endothelial cells (PAECs) cultured on the Fn coated honeycomb films in a serum free medium were characterized by using immunofluorescencet labeling of vinculin and focal adhesion kinase autophosphorylated at the tyrosine residue 397 (pY 397 FAK). Fn adsorbed around the pore periphery of a honeycomb film to form fibriller aggregates in a ring-shape structure. The sites of pY 397 FAK and vinculin were overlapped and agreed well with the adsorption site of Fn fibrils. This demonstrated that PAECs adhered onto the honeycomb films at focal contact points localized around pore periphery. The expression of pY397FAK determined by an immunoprecipitation method was 3 times higher than that on a PCL flat film as a reference. These results imply that the signaling mediated by a integrin receptor-Fn binding were activated on honeycomb films and this type of signaling was activated effectively on a honeycomb film compared with on a flat film. The cell response to honeycomb films (adhesion pattern and phosphorilation of FAK) was supposed to originate from the regularly arraigned adsorption pattern of Fn determined by the pore structure of the film.

収録刊行物

  • 表面科学 = Journal of The Surface Science Society of Japan  

    表面科学 = Journal of The Surface Science Society of Japan 27(9), 502-510, 2006-09-10 

    The Surface Science Society of Japan

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各種コード

  • NII論文ID(NAID)
    10018051591
  • NII書誌ID(NCID)
    AN00334149
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    03885321
  • NDL 記事登録ID
    8090997
  • NDL 雑誌分類
    ZM35(科学技術--物理学)
  • NDL 請求記号
    Z15-379
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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