Application of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Rich Source of Peroxidase in the Spectrofluorometric Determination of Hydrogen Peroxide in Honey Samples

• MANZOORI Jamshid L.

  Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz

• AMJADI Mohammad

  Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz

• OROOJI Maghsood

  Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz

Abstract

Crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared by a simple procedure and its enzymatic activity and total protein concentration were determined. It was found that this crude extract is a rich source of peroxidase (POx) and has high specific activity. Cross-linked polyvinylpyrrolidone was used as a stabilizer in the preparation of the crude extract. The POx activity of kohlrabi crude extract did not vary for at least 2 months when deoxygenated and stored at 4°C. This extract was applied for the spectrofluorometric determination of hydrogen peroxide using homovanillic acid as a fluorogenic substrate. POx catalyzes the hydrogen peroxide oxidation of homovanillic acid to produce a dimer which shows strong fluorescence at 420 nm with excitation at 312 nm. In the optimum conditions, the calibration graph for hydrogen peroxide was linear up to 190 ng mL^-1, with a detection limit of 4.4 ng mL^-1. The relative standard deviation (RSD) was 1.48% for 50 ng mL^-1 hydrogen peroxide. The proposed method was successfully applied to the determination of hydrogen peroxide in honey. The concentration-time profile of H₂O₂ produced upon dilution of honey was studied and H₂O₂ contents of some different honeys from various areas of Iran were determined.

Journal

• Analytical Sciences

  Analytical Sciences 22 (9), 1201-1206, 2006

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204256259328
• NII Article ID: 130004441341
• NII Article ID: 10018064196
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.22.1201
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 8050766
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8050766
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.22.1201.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.22.1201/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/22/9/22_9_1201/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed