培地条件がスターツデザートピー (<I>Swainsona formosa</I>) カルスの水分状態と増殖に与える影響

• 池田 敬

  近畿中国四国農業研究センター野菜部

• タジ アクラム

  ニューイングランド大学

• 藤目 幸擴

  京都府立大学農学部

• 野口 正樹

  野菜茶業研究所果菜研究部

書誌事項

タイトル別名

• Water Status and Growth of Callus in Sturt's Desert Pea (Swainsona formosa) as Affected by Media Condition

抄録

This work was undertaken to investigate response of callus and the water status of Sturt's desert pea (Swainsona formosa) in vitro under various environmental stress conditions. Water potential of culture media ranged from -0.27MPa to -1.05MPa so that salt and osmotic stress and different concentration of sucrose could be applied to Sturt's desert pea calli grown in the tissue culture media. The percent increase in calli was highest in standard MS medium with 30g L^-1 sucrose but was not so when the water potential of medium was adjusted to the same water potential using standard MS plus mannitol. The water potential of callus tissue was almost the same as that of culture media at any range of MS and sucrose concentrations. Turgor of callus tissue did not alter significantly under any stress conditions. It is concluded that the optimum concentration of salt, sucrose and water status of medium could be estimated by this method and that standard MS concentration and its water status are suitable for the formation of Sturt's desert pea calli.

収録刊行物

• 生物環境調節

  生物環境調節 42 (3), 177-183, 2004

  日本生物環境工学会

キーワード

• isopiestic psychrometer
• mannitol
• osmoregulation

詳細情報

• CRID: 1390282680056769280
• NII論文ID: 10018078964
• NII書誌ID: AN00129558
• DOI: 10.2525/ecb1963.42.177
• ISSN: 21851018
• ISSN: 05824087
• NDL書誌ID: 7105949
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030701191
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7105949
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可