マウス上顎相当部に放射線照射した際のリンパ球組成の変化―小腸腸管上皮内リンパ球およびパイエル板リンパ球の検討―

• 石川 裕一

  日本歯科大学歯学部歯科放射線学講座

• 佐藤 太吾

  日本歯科大学歯学部歯科放射線学講座

• 玉澤 賢

  日本歯科大学歯学部歯科放射線学講座

• 代居 敬

  日本歯科大学歯学部歯科放射線学講座

書誌事項

タイトル別名

• Changes in Lymphocyte Subsets due to Local Irradiation of a Portion of the Maxilla in Mice-A Study of Intestinal Intraepithelial Lymphocytes and Peyer's Patch Lymphocytes-
• -A Study of Intestinal Intraepithelial Lymphocytes and Peyer's Patch Lymphocytes-
• ―小腸腸管上皮内リンパ球およびバイエル板リンパ球の検討―

抄録

To enrich our knowledge of the effects of therapeutic radiation on immunity organs located out of the irradiation field for oral cancer patients, we designed a study to examine the effects of local irradiation on the intestinal mucosal immune systems of laboratory animals. Mouse maxillae were exposed to a dose of 10Gy and the ratios of subsets of intestinal intraepithelial lymphocytes (i-IELs), and Peyer's patches lymphocytes and splenocytes were measured using flow cytometry. The following results were obtained: 1) In intestinal intraepithelial, the percentage of T cells, CD4⁺ SP T cells, CD8⁺ SP T cells, CD8⁺ αα T cells, or CD8⁺ αβ T cells did not show any significant change after irradiation; 2) In Peyer's patch, the percentage of T cells, CD4⁺ SP T cells and CD8⁺ SP T cells did not show any significant change after irradiation; 3) In intestinal intraepithelial and Peyer's patch, the percentage of L-selectin^- T cells and L-selectin⁺ T cells did not show any significant change after irradiation; 4) In the spleen, the percentage of L-selectin^- T cells showed a significant increase one day after irradiation, and one of the L-selectin⁺ T cells showed a significant decrease one day after irradiation. The above findings demonstrate that irradiation does not cause any significant changes in the ratios of subsets of i-IELs and Peyer's patch lymphocytes. From these findings we could conclude that irradiation to the head and neck region has little influence on the intestinal mucosal immune system, and it may suggest that a lymphocyte homing phenomenon affects the ratio of the lymphocyte subset in each immunity organ.

収録刊行物

• 歯科放射線

  歯科放射線 44 (2), 110-118, 2004

  特定非営利活動法人 日本歯科放射線学会

キーワード

• 小腸粘膜上皮内リンパ球
• パイエル板
• 放射線照射
• フローサイトメトリー

詳細情報

• CRID: 1390282679939356160
• NII論文ID: 130003919255
• NII論文ID: 10018080443
• NII書誌ID: AN00101479
• DOI: 10.11242/dentalradiology1960.44.110
• ISSN: 21856311
• ISSN: 03899705
• Web Site: https://search.jamas.or.jp/link/ui/2004285104
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可