Rapid and Sensitive Method for Evaluation of Radical-Scavenging Activity Using Peroxyl Radicals Derived from 2,2'-Azobis(2-amidinopropane) Dihydrochloride Combined with Luminol Chemiluminescence
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A rapid and sensitive method for evaluation of the radical-scavenging activity, which uses 2,2′-azo<I>bis</I> (2-amidinopropane) dihydrochloride (AAPH) as a radical generator in combination with a luminol chemiluminescence (CL) assay, was examined. The radical-scavenging activities of various analytical-grade compounds were measured by monitoring the decrease in CL. In this method (AAPH-CL), compounds that show lower CL values have stronger radical-scavenging activity. These results were compared to those measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-colorimetry analysis. They were approximately similar in pattern (r<SUP>2</SUP>=0.6043), although there were some discrepancies. The half-inhibition concentration (IC<SUB>50</SUB>) values of 11 beverages were also evaluated using AAPH-CL and DPPH-high performance liquid chromatography methods. Data from the two methods showed a correlation (r<SUP>2</SUP>) of 0.4786. We conclude that the AAPH-CL method is suitable for the evaluation of radical-scavenging activity <I>in vitro</I>, with the advantages that it is rapid to carry out, has good sensitivity, is not affected by impurities or color in the solution, and bears some similarity to cellular processes.
- Food Science and Technology International, Tokyo
Food Science and Technology International, Tokyo 11(3), 318-323, 2005-11-01
Japanese Society for Food Science and Technology