Quantitative Identification of Rice Cultivars by Real-Time PCR

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Application of a real-time PCR system for the detection of blending and the quantification of the blending ratio of rice was investigated. Quantitative measurement by real-time PCR was performed using two types of primer sets ; one was an improved rice cultivar identification kit, while the other was newly developed for the determination of total amount of rice DNA. Calculation formulae for blending ratios were developed based on standard curves of both primer sets. These formulae were verified using DNA solutions extracted from the Koshihikari containing 5% or 25% of another rice sample. Calibration errors were less than 30% of the expected values.

収録刊行物

  • Food science and technology research  

    Food science and technology research 11(3), 344-348, 2005-11-01 

    Japanese Society for Food Science and Technology

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各種コード

  • NII論文ID(NAID)
    10018084567
  • NII書誌ID(NCID)
    AA11320122
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13446606
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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