Quantitative Identification of Rice Cultivars by Real-Time PCR

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Application of a real-time PCR system for the detection of blending and the quantification of the blending ratio of rice was investigated. Quantitative measurement by real-time PCR was performed using two types of primer sets ; one was an improved rice cultivar identification kit, while the other was newly developed for the determination of total amount of rice DNA. Calculation formulae for blending ratios were developed based on standard curves of both primer sets. These formulae were verified using DNA solutions extracted from the Koshihikari containing 5% or 25% of another rice sample. Calibration errors were less than 30% of the expected values.

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詳細情報

  • CRID
    1390282679431318272
  • NII論文ID
    10018084567
  • NII書誌ID
    AA11320122
  • DOI
    10.3136/fstr.11.344
  • COI
    1:CAS:528:DC%2BD28XkvF2ns74%3D
  • ISSN
    18813984
    13446606
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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