Cloning of Myrosinase cDNA from Wasabia japonica Matsum.
Myrosinase (β-thioglucoside glucohydrolase, EC 220.127.116.11) is involved in pungency generation in wasabi (<I>Wasabia japonica</I> Matsum.). We isolated full-length cDNA (WjMY1) encoding myrosinase from the petiole of wasabi. The deduced polypeptide has 545 amino acids with a molecular weight of 62107. WjMY1 mRNA is accumulated in whole organs of the wasabi plant ; the order of expression is petiole≥rhizome>leaf. The order was found to be similar to that of the specific activity of myrosinase. This is the first report de- scribing the isolation of a myrosinase gene from wasabi.
- Food science and technology research
Food science and technology research 11(4), 412-415, 2005-12-01
Japanese Society for Food Science and Technology