Restoration by VIP of the Carbachol-Stimulated Cl〔-〕 Secretion in TTX-Treated Guinea Pig Distal Colon

• Kokubo A.

  Department of Physiology, Kitasato University School of Medicine Department of Internal Medicine, Kitasato University School of Medicine

• Yasuoka Y.

  Department of Physiology, Kitasato University School of Medicine

• Nishikitani M.

  Department of Physiology, Kitasato University School of Medicine Department of Hygiene and Public Health, Teikyo University School of Medicine

• Saigenji K.

  Department of Internal Medicine, Kitasato University School of Medicine

• Kawahara K.

  Department of Physiology, Kitasato University School of Medicine

書誌事項

タイトル別名

• Restoration by VIP of the Carbachol-Stimulated Cl- Secretion in TTX-Treated Guinea Pig Distal Colon
• Restoration by VIP of the Carbachol-Stimulated Cl− Secretion in TTX-Treated Guinea Pig Distal Colon

抄録

To determine if vasoactive intestinal peptide (VIP) restores neural activity from tetrodotoxin (TTX) blockade, we studied the effects of VIP and related agents on carbachol (Cch)-induced Cl⁻ secretion in control-isolated guinea pig distal colon and in that treated with TTX. The short circuit current (I_sc) increased dose-dependently after serosal applications of Cch (10⁻⁶–2 × 10⁻⁵ M) and VIP (5 × 10⁻⁹–10⁻⁷ M). But no additive or synergistic increase in I_sc was observed. Cch- and VIP-induced I_sc was completely abolished by a serosal application of TTX (10⁻⁶ M). However, a serosal application, not mucosal, of VIP (10⁻⁷ M) and 8-bromo-cAMP (10⁻³ M) restored the Cch-stimulated, TTX-inhibited I_sc by 113% and 75.8%, respectively. Furthermore, mucosal and serosal applications of forskolin (aden late cyclase activator) restored the I_sc by 43.9% and 65.3%, respectively. The restored I_sc was completely abolished by atropine (muscarinic receptor antagonist). These results suggest that VIP may restore the cholinergic activity by increasing the level of intracellular cAMP, and that cholinergic neuron is very likely to be responsible for the regulation of Cl⁻ secretion at neuroepithelial junctions. The exact mechanism of VIP's effect on the TTX-inhibited epithelial Cl⁻ secretion, and its possible usefulness in the treatment of TTX-induced pathophysiological conditions, remain to be determined.<br>

収録刊行物

• The Japanese Journal of Physiology

  The Japanese Journal of Physiology 55 (6), 317-324, 2005

  一般社団法人　日本生理学会

キーワード

• tetrodotoxin
• chloride secretion
• cholinergic neuron
• VIPergic neuron
• forskolin
• cAMP

詳細情報

• CRID: 1390282680019931520
• NII論文ID: 10018087100
• NII論文ID: 130004435933
• NII書誌ID: AA00691224
• DOI: 10.2170/jjphysiol.r2135
• COI: 1:STN:280:DC%2BD287ktlWktw%3D%3D
• ISSN: 18811396
• ISSN: 0021521X
• NDL書誌ID: 7870141
• PubMed: 16324225
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7870141
• Web Site: https://search.jamas.or.jp/link/ui/2007065871
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可