In vitro Callusing and Regeneration in Trifolium resupinatum : A Fodder Legume
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<i>In vitro</i> regeneration protocol was developed in <i>Trifolium resupinatum</i>, an important fodder legume in Northwestern India. Out of 6 explants (leaf, petiole, hypocotyl, cotyledon, collar and root) tried for callus induction in 2 different media, good response was observed from hypocotyl and root explants in 'A' medium. Successful regeneration was obtained from callus induced from hypocotyl, cotyledon and root in 'A' followed with subculture in 'E' medium. One of the regenerated plantlet was characterized and compared with mother plant for isozyme-banding pattern. Wide variation for isozyme banding pattern for SOD, Peroxidase and GOT enzyme indicates the regenerant to be a somaclonal variant. Thus, <i>in vitro</i> regeneration will help in widening the genetic base of the species.
CYTOLOGIA 71(3), 229-235, 2006-09-25
Japan Mendel Society, International Society of Cytology