Microfluidic Device for Sequential Injection and Flushing of Solutions and its Application to Immunosensing

• Nashida Norihiro

  Graduate School of Pure and Applied Sciences, University of Tsukuba

• Suzuki Hiroaki

  Graduate School of Pure and Applied Sciences, University of Tsukuba

Abstract

A microfluidic system with injecting and flushing functions was developed. In the system, hydrophilic flow channels have a dry-film photoresist layer which facilitates the introduction of solutions from four injection ports. The injection and flushing of solutions are controlled by valves operated by electrowetting. The valves consist of gold working electrodes in the flow channels or a through-hole in the glass substrate. Solutions can be sequentially introduced through the injection ports into a reaction chamber and flushed through a valve in the through-hole. Necessary immunoassay steps can be conducted on the chip, and a target antibody can be detected electrochemically.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 126 (5), 202-203, 2006

  The Institute of Electrical Engineers of Japan

Keywords

• electrowetting
• valve
• hydrophilic flow channel
• immunoassay

Details

• CRID: 1390282679438268544
• NII Article ID: 10018111826
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.126.202
• ISSN: 13475525
• ISSN: 13418939
• Web Site: http://www.jstage.jst.go.jp/article/ieejsmas/126/5/126_5_202/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed